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A) Anti-Ca V 2.2 antibody validation. B and C ) Ca V 2.2 protein levels in brain of WT, +18a- and Δ18a mice. C) Mean (filled symbols) ± s.e.m and data from individual mice (empty symbols). F = females, M = males.
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A) Anti-Ca V 2.2 antibody validation. B and C ) Ca V 2.2 protein levels in brain of WT, +18a- and Δ18a mice. C) Mean (filled symbols) ± s.e.m and data from individual mice (empty symbols). F = females, M = males.
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A) Anti-Ca V 2.2 antibody validation. B and C ) Ca V 2.2 protein levels in brain of WT, +18a- and Δ18a mice. C) Mean (filled symbols) ± s.e.m and data from individual mice (empty symbols). F = females, M = males.
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A) Anti-Ca V 2.2 antibody validation. B and C ) Ca V 2.2 protein levels in brain of WT, +18a- and Δ18a mice. C) Mean (filled symbols) ± s.e.m and data from individual mice (empty symbols). F = females, M = males.
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A) Anti-Ca V 2.2 antibody validation. B and C ) Ca V 2.2 protein levels in brain of WT, +18a- and Δ18a mice. C) Mean (filled symbols) ± s.e.m and data from individual mice (empty symbols). F = females, M = males.
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A) Anti-Ca V 2.2 antibody validation. B and C ) Ca V 2.2 protein levels in brain of WT, +18a- and Δ18a mice. C) Mean (filled symbols) ± s.e.m and data from individual mice (empty symbols). F = females, M = males.
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A) Anti-Ca V 2.2 antibody validation. B and C ) Ca V 2.2 protein levels in brain of WT, +18a- and Δ18a mice. C) Mean (filled symbols) ± s.e.m and data from individual mice (empty symbols). F = females, M = males.

Journal: bioRxiv

Article Title: Cacna1b alternative splicing is linked to associative learning

doi: 10.64898/2026.02.17.706441

Figure Lengend Snippet: A) Anti-Ca V 2.2 antibody validation. B and C ) Ca V 2.2 protein levels in brain of WT, +18a- and Δ18a mice. C) Mean (filled symbols) ± s.e.m and data from individual mice (empty symbols). F = females, M = males.

Article Snippet: Next, membrane was blocked for 1 h with 5% BSA in PBS-T at RT and washed three times for 3 min in PBS-T. Anti-Ca v 2.2 primary antibody (Alomone Rabbit anti-Ca v 2.2, #ACC-002) was prepared 1:100 with 1% BSA in PBS-T and 15 μL 10 % NaN 3 for a final concentration of 0.425 μg/mL.

Techniques: Biomarker Discovery